Malonic acid monoamides as effective bactericide inhibitors

To protect oilfield equipment and pipelines from general and microbiological corrosion, the development of bactericide-inhibitors of complex action is required. Forming a shielding layer on the metal surface, the inhibitor isolates the surface from the aggressive environment, thereby reducing the corrosion rate. For this purpose, malonic acid (MA) monoamides were synthesized and their antimicrobial properties were studied. To determine the bactericidal properties of inhibitors in the experiment, strain 1143 of the species «Desulfovibrio desulfuricans» of sulfate-reducing bacteria (SRB) was used. These bacteria generally multiply extensively in Postgate, but the most suitable for this strain is Postgate B. At the first stage of the experiment in pre-sterilized test tubes with a volume of 20 ml, it was determined that the number of bacteria in the medium without inhibitor is n=108. For this, the diluted bacteria were kept at a temperature range of 30…32 °C for 7…14 days. Further, aqueous solutions (with a concentration of 50, 150, and 250 mg/l) prepared on the basis of MA monoamides were added to the test tubes with bacteria. The presence of SRB in the medium is determined as follows: after adding the reagent solutions to the test tubes with the Postgate B solution, they were placed in a thermostat at 30…32 °C. The bactericidal effect of the reagents on the functioning of the SRB was studied mainly by observing for 15 days and then calculating the amount of H2S formed. The formation of H2S was determined by iodometric titration of the test samples according to OST 39-234-89. Studies have shown that solutions of the synthesized MA monoamides at a concentration of 50, 100, 150 mg/l have, a 100% bactericidal effect, reducing the number of SRBs from 108 to zero, thereby showing more effective results than industrial inhibitors currently used.
Thus, MA monoamides can be recommended as bactericidal inhibitors against SRB.

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